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上海联硕生物科技有限公司
GelRed染料

GelRed染料

商家询价

产品名称： GelRed染料

英文名称： GelRed Nucleic Acid Gel Stain, 10,000X in water

产品编号： 41003

产品价格： 0

产品产地： 美国

品牌商标： Biotium

更新时间： null

使用范围： null

上海联硕生物科技有限公司
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 GelRed染料

GelRed染料     GelRedTM Nucleic Acid Gel Stain, 10,000X in water biotium 41003

  • GelRedTM is an ultra sensitive, extremely stable and environmentally safe fluorescent nucleic acid dye designed to replace the highly toxic ethidium bromide (EB) for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. GelRed is far more sensitive than EB without requiring a destaining step (Figure 1). GelRed and EB have virtually the same spectra (Figure 3), so you can directly replace EB with GelRed without changing your existing imaging system.

     

    GelRedTM can be used to stain dsDNA, ssDNA or RNA in agarose gels via either precast or post gel staining. GelRed can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gels via post gel staining. GelRed is also compatible with downstream DNA manipulations such as restriction digest, sequencing, and cloning.

    A series of safety tests have confirmed that GelRedTM is noncytotoxic, nonmutagenic and nonhazardous at concentrations well above the working concentrations used in gel staining. As a result, GelRed can be safely disposed of down the drain or in regular trash, providing convenience and reducing cost in waste disposal. For detailed test results, you may download a complete GelRed/GelGreen Safety Report here.

    For more information, please see GelRed and GelGreen Frequently Asked Questions.

    Biotium offers GelRedTM 10,000X solution in water, our latest formulation that eliminates the hazards of handling DMSO for better safety. We continue to offer GelRedTM 10,000X solution in DMSO (cat#41002) for established users of GelRedTM in DMSO who do not wish to change their laboratory protocols. The performance and stability of GelRedTM 10,000X is comparable in both the water and DMSO formulations. For your convenience, we also offer ready-to-use GelRedTM 3X in water (cat#41001) that can be directly used for post gel staining. For customers who look for large pack size, we offer a cost-saving bulk pack size of 10mL (cat#41003-1).

    For related products, download our Genomics and Proteomics Products Brochure.

    FEATURES

    • Safer than EB

      • Shown by the Ames test and other tests to be nonmutagenic and noncytotoxic

    • Easy disposal

      • Passed environmental safety tests for direct disposal down the drain or in regular trash

    • Ultra-sensitive

      • Much more sensitive than EtBr and SYBR Safe

    • Extremely stable

      • Available in water, stable at room temperature for long-term storage and microwavable

    • Simple to use

      • Very simple procedures for either precast and post gel staining

    • Perfectly compatible with a standard UV transilluminator

      • GelRed replaces EtBr with no optical setting change; GelGreen replaces SYBR or GelStar with no optical setting change (see Figure 3)

    • Perfectly compatible with downstream applications

      • Compatible with downstream DNA manipulations such as restriction digest, cloning, and sequencing.
    The Most Sensitive and Stable Precast Gel Stain
    Figure 1.GelRedTM is significantly more sensitive than ethidium bromide (EB) for detecting low-level DNA, especially in the lower molecular weight area. Shown left are two-fold serial dilutions of 1 Kb Plus DNA Ladder from Invitrogen electrophoresed on 1% agarose gels precasted with GelRed or EB in 1x TBE. The total amount of DNA loaded per lane was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. Gels were imaged using 300-nm transillumination and photographed with an EB filter and Polaroid 667 black-and-white print films.

     

    The Most Sensitive and Stable Post Gel Stain
     
    Figure 2. GelRedTM displays consistently superior sensitivity for post gel staining, regardless of the filter used (A vs. C) and storage and handling condition. SYBR Gold, however, showed comparable performance only when used fresh from the manufacturer and with a SYBR filter (B vs. D). Following a few freeze-thaw cycles, SYBR Gold 10,000X solution degraded significantly, resulting in poor staining (E). SYBR Gold 1X solution also degrades over time (see Figure 4). Two-fold serial dilutions of 1kb Plus DNA Ladder from Invitrogen were electrophoresed on 1% agarose gels in 1x TBE and post- stained with GelRedTMand SYBR Gold, respectively. Gels were imaged using 300-nm transillumination and photographed with the indicated filters and Polaroid black-and-white print films. The total amount of DNA per lane for each serial dilution was: 200 ng, 100 ng, 50 ng and 25 ng from left to right. 

     

    Figure 3. Excitation and emission spectra of GelRed and GelGreen in the presence of DNA in PBS buffe

     

    Note: *GelRed and its uses are covered by US patent numbers 7960498, 7803943, and 8232050. **SYBR is trademark of Molecular Probes, Inc. and GelStar is trademark of FMC corporation.

    Please also see our EvaGreenTM(cat#31000), a breakthrough nucleic acid dye ideally suited for quantitative real-time PCR(qPCR). By incorporating a smart "release-on-demand" DNA-binding technology, EvaGreenTM has low PCR inhibition while exhibiting superior sensitivity. Similar to our GelRedTM, EvaGreenTM has remarkable stability.

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